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Fig. 9 | Frontiers in Zoology

Fig. 9

From: Immunohistochemical and ultrastructural properties of the larval ciliary band-associated strand in the sea urchin Hemicentrotus pulcherrimus

Fig. 9

Immunohistochemistry of cytoplasmic tubulins in the ciliary band (CB)-associated strand (CBAS). a Immunoblotting of the 6-arm plutei lysate with mouse anti-β-tubulin monoclonal antibody (mAb) (lane 1), mouse anti-acetylated α-tubulin mAb (lane 2), mouse anti-α-tubulin mAb (lane 3), and mouse pre-immune serum (lane 4). Arrow indicates the 63-kDa region. bh Confocal laser scanning micrographs. Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue). b One-μm thick single optical longitudinal cross-section of the triple-stained CBAS. Double-arrow, cilia; red arrow, the CBAS positive to anti-GAD pAb; white arrow, cytoplasmic β-tubulin (green). ce and g; apical surface views of the larval arms. c Twelve-μm thick stacked image of the CBAS (red arrow). d, e Color channel arranged images of (c). d Twelve-μm thick stack image of the CBAS stained with anti-GAD pAb (red arrow). e Twelve-μm thick stack image stained with anti-β-tubulin mAb (green). f Triple-stained 21-μm thick longitudinal stack image of optical cross sections of larval arm. The CBAS was stained with anti-GAD pAb (red). Yellow arrow, cytoplasmic α-tubulin (green). g Triple-stained 28-μm thick stack image of optical cross sections of the CBAS that was stained with anti-GAD pAb (red, red arrow). CB cilia were stained with mouse anti-acetylated α-tubulin (green). White arrow, CB cilia. h Fourteen-μm thick stack image of optical cross sections of mouse pre-immune serum-treated (green, not shown) larval arm. Blc, blastocoel; Ep, epithelium of the larval arm

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