Fig. 8

Immunochemical detection of synaptophysin (Syn) in the ciliary band-associated strand (CBAS). (a) Immunoblotting of the 6-arm plutei lysate was probed with rabbit anti-sea urchin-Syn pAb (lane 1) and detected a single band in the 30-kDa region (arrow). Lane 2: Rabbit pre-immune serum. (b–e) Confocal laser scanning micrographs of whole-mount immunohistochemistry of a 6-arm pluteus. (b) Left side view of the pluteus. The CBAS was positive for Syn (green) and the 5HT receptor (5HThpr; red). (c–e) High-magnification of the CBAS indicated by box (c–e) in (B). (c) Syn expression. (d) 5HThpr expression. (e) Merged image between (c) and (d) with nuclei stained with 4′,6-diamidino-2-phenylindole (blue). Green arrows; apical protrusions from the CBAS; white arrows, perikarya; yellow arrow, granular expression of Syn in the axon-like region of the CBAS