Fig. 7

Immunochemical detection of encephalopsin (ECPN) in the ciliary band-associated strand (CBAS). (a) Immunoblotting of the 4-arm plutei lysate. Lane 1, rabbit anti-Hp-ECPN pAb; Lane 2, rabbit pre-immune serum. Arrow, 76 kDa region. (b–) Confocal laser scanning micrographs of whole-mount immunohistochemistry of 4-arm pluteus. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue). (b) Triple-stained aboral view of 4-arm pluteus. (c–e) High-magnification image of a postoral arm indicated by box (c–e) in (B). (c) ECPN (green) at the CBAS. (d) 5HThpr (red) at the CBAS. (e) Merged image between (c) and (d). (f) Arm of a 6-arm pluteus triple-stained with rabbit pre-immune serum (green, negative), mouse anti-5HThpr (red), and DAPI. Arrows in (c–f), perikarya of the CBAS; Bcl, blastocoel