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Fig. 3 | Frontiers in Zoology

Fig. 3

From: Immunohistochemical and ultrastructural properties of the larval ciliary band-associated strand in the sea urchin Hemicentrotus pulcherrimus

Fig. 3

Immunohistochemical cell surface property of the ciliary band-associated strand (CBAS). (a) Schematic of arm tip region indicated by box (Fig. 3) in Fig. 2a. Red line, CBAS with perikarya (red dots). Vertical bar (b–l), approximate region of image (b–l). (b) Glutamate decarboxylase (GAD) expression in the CBAS. (c) Epith-2 expression in the perikaryon (green arrow). (d) Merged image between (b) and (c). (e) Hybrid Super Resolution software (HSR)-processed image of (d) shows locations of the optical cross-sections (dotted lines). (fj) Optical cross-sections indicated by vertical dotted lines (f–j) in (e). (k) HSR-processed 3D reconstructed image of the CBAS. (l) The other side of image (k) after rotation mostly covered by the Epith-2 signal. (m) Schematic of cross-section of arm indicated by bar (m) in (a). Bcl, blastocoel. Nuclei in (d–l) were stained with 4′,6-diamidino-2-phenylindole (blue). Aboral, aboral ectoderm regiuon; CB, ciliary band area; green arrow, perikaryon; Oral, oral ectoderm region; red arrow, axon-like region; white arrow, apical protrusion

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