Table 2 Species-specific quantitative PCR (qPCR) primers
Species | Gene | Direction | Sequence | Amplicon size (bp) |
|---|---|---|---|---|
Golden-collared Manakin | SRD5A1 | Forward | CCAAGAGGAGGAATGTTTGAG | 142 |
Reverse | CCCTTGAACCCAAGATGAAA | |||
SRD5A2 | Forward | AAGATTCCTCAAGGGGGACTG | 132 | |
Reverse | GCAAAGAGTGAAAAAGGCAAAG | |||
SRD5B1 | Forward | TTTACTGTGGCAAGCTGTGG | 75 | |
Reverse | TCTTCAGTGTCTTCTCCAGTGTG | |||
Zebra Finch | SRD5A1 | Forward | AGGCCGAGCTTACCACCTAT | 68 |
Reverse | ACCTGTAATGAAGCGCAAGC | |||
SRD5A2 | Forward | GGCCTCCTCTTATTTCTGCTG | 108 | |
Reverse | TCCTCCTTGGGGAATCTTG | |||
SRD5B1 | Forward | CTTTCAAGCCTGGAGATGCAC | 83 | |
Reverse | CCAAGTGGCACATAAGTCTGTC |