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Fig. 8 | Frontiers in Zoology

Fig. 8

From: Skeletal regeneration in the brittle star Amphiura filiformis

Fig. 8

Afi-c-lectin expression (green) combined with EdU labeling (red) and counterstained with nuclear stain DAPI (blue) showing that skeletogenic cells do not proliferate. A) Maximum projection of proximal segments of 50 % regenerating arm. A’ and A”) Single z-planes of A showing Afi-c-lectin expressing cells do not overlap with EdU-labeled cells. Yellow dashed box shows one case of yellow signal suggesting potential overlap of red and green signals. B) Magnified image in yellow dashed box from A showing cells in z-plane number 3 and z-plane number 15. The EdU labeled red nucleus is seen clearly in z-plane 3 where Afi-c-lectin expression is very faint, whereas on z-plane 15 where the whole cytoplasm of the green-labeled Afi-c-lectin cell is clearly visible, the nucleus of this cell is labeled with DAPI but not EdU (asterisk). C) Maximum projection of distal-most end of 50 % regenerating arm. White dashed half-circle indicates position of terminal ossicle. C’ and C”) Single z-planes of C showing Afi-c-lectin expressing cells do not overlap with EdU-labeled cells. Yellow dashed box shows one case of yellow signal suggesting potential overlap of red and green signals. D) Magnified image in yellow dashed box from C showing cells in single z-plane. Again the nucleus of the Afi-c-lectin expressing cell is labeled with DAPI (asterisk) and is in close proximity to the EdU-labeled nucleus causing the yellow overlap signal

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