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Fig. 5 | Frontiers in Zoology

Fig. 5

From: Skeletal regeneration in the brittle star Amphiura filiformis

Fig. 5

Afi-c-lectin expression during early arm regeneration stages. a) Schematic representation of a 3/4 stage regenerate. b–d) Chromogenic whole mount in situ hybridization (WMISH) showing expression of gene at stages 2 and 4. b) Afi-c-lectin is expressed at stage 2 in a broad, sub-epidermal domain. c) At stage 4 the expression of Afi-c-lectin becomes much more restricted to the sub-epidermal domain. d) Higher magnification of arm in c. e, f Fluorescent in situ hybridization of sections through stage 3/4 arm counterstained with the nuclear marker DAPI. e) Frontal paraffin section showing fluorescent ISH of Afi-c-lectin counterstained with DAPI. Afi-c-lectin is clearly localized to single cells just beneath the epidermis. Asterisk marks cells in sub-epidermal layer seen from oral view, due to the slanted plane of sectioning along the oral-aboral axis. f) Higher magnification of arm in e. Scale bars - 50μm

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