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Fig. 1 | Frontiers in Zoology

Fig. 1

From: Skeletal regeneration in the brittle star Amphiura filiformis

Fig. 1

Early stages of arm regeneration in the brittle star Amphiura filiformis. A) Schematic diagram of early arm regeneration stages and average timing to those stages, based on morphological landmarks. Stage 1-wound healing and re-epithelialization. Stage 2-regenerative bud formation. Stage 3-appearance of the radial water canal, coelomic cavities (aboral coelomic canal, ectoneural and hyponeural sinuses) and radial nerve in the regenerative bud. Stage 4-appearance of first metameric units (arm segments). Stage 5-advanced extension of arm, formation of several metameric units at proximal end. B–E) Oral view of fixed arms at stages 2 (B), 3 (C), 4 (D) and 5 (E) shown using differential interference contrast (DIC) microscopy. C’) Detail of C focusing on dermal layer. D’) Detail of D. E’) Detail of E. Arrows - the dermal layer, asterisk - cuticle, D - distal, L - left, OV - oral view, P - proximal, R - right, RWC - radial water canal, S - spicule

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