Fig. 7
From: Neural architecture of Galathowenia oculata Zach, 1923 (Oweniidae, Annelida)
Peripheral nervous system in the trunk of Galathowenia oculata. Anti-acetylated α-tubulin (green, cyan) and anti-serotonin (red) immunoreactivity (lir) nuclei labeling with DAPI (blue), maximum projections, whole-mount specimens. Ventral to the left, anterior to the top. a Main tubulin-lir longitudinal neurite bundles up to the 5th chaetiger, dorsolateral view. b Serotonin-lir lateral neurite plexus in the 2nd, 3rd and 4th chaetigers, lateral view. c The same fragment as in B. Main tubulin-lir lateral longitudinal neurite bundles and numerous serially arranged circular neurite bundles. d, e Serotonin-lir and tubulin-lir circular neurite bundles and plexuses between the main longitudinal neurite bundles, the 5th chaetiger, lateral view. Arrows point to ramifying of neurite bundles. f Serotonin-lir somata of the ventral nerve cord and main longitudinal neurite bundles, the 6th chaetiger, lateral view. g, h Serotonin-lir and tubulin-lir circular neurite bundles in the region of the parapodia, posterior portion of the 5th chaetiger, lateral view. i Serotonin-lir and tubulin-lir circular neurite bundles in the posterior-most segments (brackets mark tubulin-lir neurite bundles in each segment), ventrolateral view. j Tubulin-lir longitudinal neurite bundles in the posterior-most segments, lateral view. k Serotonin-lir somata associated with the neurite plexus and the main lateral longitudinal neurite bundles, lateral view. l Main serotonin-lir dorsal neurite bundle associated with the dorsal neurite plexus, dorsolateral view. cc – circumesophageal connectives, cib – ciliary bands, cil – cilia, dc – dorsal commissure, dn – main dorsal neurite bundle, dnn – dorsal neurite plexus, lgl – autofluorescent glands of lateral band, lln – left main lateral neurite bundle, ln – main lateral neurite bundle, lnn – lateral neurite plexus, lns – immunopositive somata associated with the main lateral neurite bundles, nc – notopodia capillaries, nu – neuropodial uncini, op – nephridiopore, py – pygidium, pyn – pygidial neurite bundles interconnecting the ventral and dorsal neurite bundles, rln – right main lateral neurite bundle, scn – serotonin-lir serially arranged circular neurite bundles, tcn – tubulin-lir serially arranged circular neurite bundles, vcs – serotonin-lir somata associated with ventral nerve cord, vnc – ventral nerve cord. Legend for cover image: Medullary brain commissure of Galathowenia oculata (Oweniidae; Annelida) showing cell nuclei by DAPI staining (blue) and FMRFamide-like immunoreactivity (yellow)