Fig. 4

FMRFamide-like immunoreactive (−lir) nervous system in the head region. Anti-FMRFamide-lir (yellow) and nuclei labeling with DAPI (blue), maximum projections, whole-mount specimens. a Overview of FMRFamide-lir nervous system in the head region and the anterior-most segments, lateral view, ventral side is to the left. b, c FMRFamide-lir neural elements in the head region: the dorsal commissure and the circumesophageal connectives, dorsal and ventral views, respectively. Arrows indicate the position of eyes. e shows precise details of dorsal neural elements. d FMRFamide-lir somata of the head collar, lateral view. Immunocytochemically positive (ICC-positive) somata are distributed along the dorsal commissure within the head collar and around the eyes, ventral side is to the right. e Somata whose processes interconnecting the dorsal commissure and the main dorsal neurite bundle, dorsal view of the head collar (larger view of b). f Color coded image of ICC-positive somata around the eyes, lateral view of the head collar (the same specimen as in a). cc - circumesophageal connectives, ccc - neurites interconnecting cc, cs – ICC-positive somata along the dorsal commissure, dc - dorsal commissure, dn – main dorsal neurite bundle, dns – ICC-positive somata whose processes interconnect dc and dn, e – eye, es – large ICC-positive somata around the eyes, hc – head collar, hs – ICC-positive somata located in the epidermis of the anterior head region, vlb – main ventrolateral neurite bundle, vnc – ventral nerve cord, vpo – ventral pharyngeal organ