Fig. 1
From: β carbonic anhydrase is required for female fertility in Drosophila melanogaster
β-CA gene expression level is reduced in RNAi knockdown flies (+Mif) compared to control flies (−Mif). β-CA RNAi lines were crossed over the GS-tub-GAL4 line and the eclosed F1 generation progeny was grown for 6 days at +29 °C in food with Mif (knockdown flies, +Mif) or without Mif (control flies, −Mif). Total RNA was extracted from 3 x 3–5 females and males of the lines indicated. Results are shown as % of expression of the control lines, which were normalized to 100 % each. ** = p < 0.01, *** = p < 0.001. β-CA RNAi1/GS-tub-GAL4 females ctrl vs. RNA-induced: t stat = 3.63, df = 9; β-CA RNAi1/GS-tub-GAL4 males ctrl vs. RNA-induced: t stat = 12.15, df = 9; β-CA RNAi2/GS-tub-GAL4 females ctrl vs. RNA-induced: t stat = 6.13, df = 10; β-CA RNAi1/GS-tub-GAL4 males ctrl vs. RNA-induced: t stat = 7.48, ds = 10.