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Fig. 8 | Frontiers in Zoology

Fig. 8

From: Nervous system development in lecithotrophic larval and juvenile stages of the annelid Capitella teleta

Fig. 8

aTUB-LIR and 5HT-LIR in 7-day old C. teleta juveniles. Images are z-stack confocal images of 7-day old juveniles labeled with anti-acetylated-α-tubulin (green), anti-serotonin (red) and TO-PRO-3 nuclear stain (blue). Panels with the same letter are different focal planes of the same animal. Merged images (a, b) are z-stacks that begin below the surface, while the single-channel images (a’, a”, b’, b”) begin at the surface. a’ and b’ are single-channel images of anti-acetylated-α-tubulin labeling while a” and b” are single-channel images of anti-serotonin labeling. The scale bar in A is 50 μm, and all images are to approximately the same scale. The pharynx (px) and esophagus (es) are labeled in a. Brackets in aa” and bb” mark the boundaries of segment (seg) 11. Segment 3 is also bracketed in bb”. In a’ and b’, an open arrowhead marks the minor abdominal peripheral nerve in segment 11, and the asterisk and filled circle indicate the two main peripheral nerves in segment 11. In a’, a closed arrowhead points to the left nuchal organ, an open arrow indicates the first branchpoint in the left anterior enteric nerve (aEN), and the left ventral-posterior enteric nerve (vpEN) is indicated. In b’, closed arrowheads point to the nuchal organs, and open arrows indicate the metanephridia. Stage is indicated in the lower-left corner, and view is indicated in the lower-right corner. All lateral views are of the left side. Anterior is to the left in all views. es, esophagus; lat, lateral; px, pharynx; vent, ventral

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