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Fig. 5 | Frontiers in Zoology

Fig. 5

From: Nervous system development in lecithotrophic larval and juvenile stages of the annelid Capitella teleta

Fig. 5

aTUB-LIR and 5HT-LIR in late-stage C. teleta larvae (stages 7 – 9). Images are z-stack confocal images of larvae labeled with anti-acetylated-α-tubulin (green), anti-serotonin (red) and TO-PRO-3 nuclear stain (blue). Panels labeled with an apostrophe (e.g., a') are single-channel images of either aTUB-LIR (’) or 5HT-LIR (”) from the merged image without an apostrophe (e.g., a) except where otherwise noted. The scale bar in a is 50 μm, and all images are to approximately the same scale except where otherwise noted. c is a cropped, 2.1x magnified image of the brain. In a, an open arrowhead points to the prototrochal nerve ring. The open arrow in a’ points to a dorsal bundle of neurites that extend from the brain neuropil. In a’ and b, a closed arrowhead marks the position of a dorsal split in the circumesophageal connective. An open arrowhead in b, c, f and g points to the scac+. The open arrow in c and g indicates a bundle of neurites that extend to the surface. The position of the prototroch (pt) and telotroch (tt) is indicated in d, e, f, and g. In e, the open arrowheads mark the right pair of metanephridia, and the closed arrows point to ventral-lateral, longitudinal neurites. Lines in e and g indicate the approximate thoracic-abdominal boundary. In g, the dorsal pad of the pharynx is labeled with a “px”. Stage is indicated in the lower-left corner, and view is indicated in the lower-right corner. All lateral views are of the left side. Anterior is to the left in all ventral, dorsal and lateral views, and ventral is down in all anterior views. ant, anterior; dors, dorsal; lat, lateral; pt, prototroch; px, pharynx; tt, telotroch; vent, ventral

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