Fig. 3
From: Positional RNA-Seq identifies candidate genes for phenotypic engineering of sexual traits
In situ hybridization (ISH) screen of positional candidate transcripts in Macrostomum lignano. ISHs of transcripts of different classes (see main text and Table 1). (a-c) Class [0,0,0]: non-differentially expressed transcripts (red box). (d-i) Class [+,0,0]: testis region-specific transcripts (blue box). (j-n) Class [+,+,0]: ovary region-specific transcripts (purple box). (o) Class [0,+,0]: ovary-region specific transcripts (orange box). (p-r) Class [0,0,+]: tail region-specific transcripts (green box). (s) Class [0,+,+]: belonging to “other classes” (black box). Note the level of the testes (black triangles) and ovaries (white triangles), and the nonspecific background staining (*). Eggs at advanced developmental stages appear yellow or orange in the specimens