Figure 2From: Sparing spiders: faeces as a non-invasive source of DNA Gel image of the newly developed multiplex PCR systems. QIAxcel gel image of PCR products generated with the multiplex PCR system LIN and the duplex PCR system DUP. LIN: Diplocephalus helleri (D.h.; 151Â bp), Erigone tirolensis (E.t.; 186Â bp), Janetschekia monodon (J.m.; 240Â bp), Agyneta nigripes (A.n.; 264Â bp), Entelecara media (E.m.; 298Â bp), artificial mixes containing 300 and 200 double stranded (ds) templates per target, respectively. DUP: Pardosa nigra (P.n.; 118Â bp), Pardosa saturatior (P.s.; 202Â bp), artificial mixes containing 3750 and 375 ds templates per target species, respectively. An internal marker is run alongside each sample (15 and 3000Â bp), and the scale on the left and right side enables an estimation of fragment length. At higher template DNA concentrations, D.h. and J.m. may produce an additional amplicon of ~390 and ~400Â bp, respectively.Back to article page