Gel image of the newly developed multiplex PCR systems. QIAxcel gel image of PCR products generated with the multiplex PCR system LIN and the duplex PCR system DUP. LIN: Diplocephalus helleri (D.h.; 151 bp), Erigone tirolensis (E.t.; 186 bp), Janetschekia monodon (J.m.; 240 bp), Agyneta nigripes (A.n.; 264 bp), Entelecara media (E.m.; 298 bp), artificial mixes containing 300 and 200 double stranded (ds) templates per target, respectively. DUP: Pardosa nigra (P.n.; 118 bp), Pardosa saturatior (P.s.; 202 bp), artificial mixes containing 3750 and 375 ds templates per target species, respectively. An internal marker is run alongside each sample (15 and 3000 bp), and the scale on the left and right side enables an estimation of fragment length. At higher template DNA concentrations, D.h. and J.m. may produce an additional amplicon of ~390 and ~400 bp, respectively.