Figure 3

Feeding in control and MIP knockdown larvae. (A) Timecourse of initiation of feeding during Platynereis larval development. % larvae feeding on Tetraselmis marina algae between 5 – 14 dpf, n = 3 x 30 larvae. (B) % larvae feeding at 7 - 9 dpf following injection of MIP mismatch (n = 210 larvae mismatch MO1, 118 larvae mismatch MO2) or start (n = 424 larvae start MO1, 79 larvae start MO2) morpholinos (C) % larvae feeding at 10 - 12 dpf following injection of MIP mismatch (n = 115 larvae mismatch MO1, 72 larvae mismatch MO2) and start (n = 185 larvae start MO1, 62 larvae start MO2) morpholinos. Data in (A-C) are shown as mean +/- s.e.m. p-value cut-offs based on unpaired t-test: *** <0.001; ** < 0.01; * <0.05. (D–G) Anterior view of 6 dpf Platynereis injected with MIP mismatch or start morpholinos and immunostained with Platynereis MIP antibody (red) counterstained with acetylated tubulin (grey). Identical confocal microscopy and image processing parameters were applied to all images. Scale bar: 100 μm. Abbreviations: mism, mismatch; MO, morpholino; a-acTub, anti acetylated tubulin.