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Table 7 Primers of 21 genes used for RT-qPCR

From: Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2

Primer ID

Protein description

Primer sequence

Primer efficiency

Cellular stress/heat shock response

   

HSP 26

Heat shock protein 26

F_AGGCAAGAGGCCGACAGA

1.98

B_AAGCGGCGGTTGAAACG

HSP 60

Heat shock protein 60

F_GCCAACGGCACCTTCGT

1.99

B_CCTTGGTGGGATCGATGATT

HSP 70_1

Heat shock protein 70

F_AGCACTTCGTCGGCTAAGGA

2.01

B_CCTGGGCAGATGATGAAAGAG

HSP 70_2

Heat shock protein 70

F_GTGTGGGCGTGTTCAAGAATG

2.03

B_CGGTTGCCCTGGTCGTT

HSP 70_3

Heat shock protein 70

F_CAACGTGCTCATCTTCGATCTG

1.98

B_CTCGATGGTCAGGATGGATACA

HSP 70_4

Heat shock protein 70

F_CCAACATGTCGGGAGAGATGA

2.00

B_CATGAGCGTTCCCCTAGGAA

HSP 90

Heat shock protein 90

F_GACACATCCACCATGGGATACA

2.03

B_TGCTGTGGTCTGGGTTGATC

Acid–base regulation

   

CA

Carbonic anhydrase

F_TACGTGTCGGCCGATAGCA

1.92

B_AAAGTCCGACCCGCTTCAC

NBC

Sodium bicarbonate cotransporter

F_CCGCCGTCATTGTCAACAG

2.01

B_TGGTATCCGCCACCCTTCT

NKA

Sodium potassium ATPase

F_CCCCGAGAGGATCCTTGAAC

2.06

B_AGGCTTCTCCTCGCCATTC

NKCC

Sodium potassium chloride cotransporter

F_GGGCAAGGACATCAGAAAGG

1.96

B_TCTACTTCACGGCGGAGCTT

NHE

Sodium hydrogen exchanger

F_GCGGAGACCTGCTGGCTAT

1.99

B_CGACTTGGCTAACACGTATTGG

VA

V1-ATPase

F_CACCCCATCCCCGATCTC

1.96

B_CTGCCGCTCCACGTAGATTT

Mitochondrial energy metabolism

   

atpA

ATP synthase

F_GGTGAATACTTCCGCGACAAC

1.96

B_TGCTTGGACAGATCGTCGTAGA

CCR

Cytochrome C reductase

F_GATCAGACCCAGACCAGTCCTT

1.99

B_CATAGCGCCGGAGGTGTT

COX

Cytochrome C oxidase

F_CGCTGCAGATGTTATTCACTCAT

1.99

B_TCCAGGGATAGCATCAGCTTTT

IDH

Isocitrate dehydrogenase

F_TGGCTCAAAAGAGGACCTATGCA

1.95

B_CCACCACCGGGTTCTTCAC

NAD

NADH dehydrogenase

F_CCCATAATTAACATCTCGGCAA

1.98

B_CTGCCCACATTGATTTAGCTTTT

SDH

Succinate dehydrogenase

F_CTCCGAGGAGAGGCTCAAGA

2.02

B_GGTGTGGCAGCGGTATACG

PDH

Pyruvate dehydrogenase

F_CTGGACGAGGAGACCATCGT

2.02

B_TCCACCGTCACCAGGTTGT

Potential housekeeping

   

Tub

Tubulin

F_GAGGACGCGGCCAACA

2.03

B_GACAATTTCCTTGCCGATGGT

  1. Genes were classified according to their function into cellular stress/heat shock response, acid–base regulation and mitochondrial energy metabolism.