Skip to main content

Table 3 Gene expression analysis: gene expression (quantities) in zoea and megalopa larvae of Hyas araneus at different time points in development classified according to their function in acid-base regulation

From: Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2

 

Acid-base regulation

 

Zoea I day 0

Zoea I day 15

Zoea II day 3

Zoea II day 15

Megalopa day 3

Gene

C

C

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

10°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

 

CA

1.59±0.26

2.06±0.39

1.50±0.14

1.78±0.24

1.52±0.18

1.65±0.11

 

1.31±0.10

1.58±0.07

1.10±0.08

 

1.23±0.06

2.20±0.09

1.55±0.08

1.74±0.08

1.62±0.06

1.35±0.06

0.99±0.10

1.26±0.07

1.24±0.05

NaK

1.30±0.31

1.74±0.34

1.64±0.09

1.51±0.25

1.55±0.15

1.26±0.10

 

1.35±0.07

1.46±0.09

0.96±0.05

0.95±0.03

1.79±0.10

1.34±0.14

1.59±0.11

 

1.43±0.12

1.27±0.09

1.32±0.08

 

1.16±0.10

1.17±0.06

 

NBC

1.24±0.30

2.04±0.37

1.82±0.08

1.44±0.20

1.87±0.15

1.24±0.10

1.25±0.09

1.65±0.04

1.15±0.05

 

1.51±0.04

1.90±0.34

0.89±0.05

1.02±0.18

0.88±0.04

1.29±0.05

1.49±0.06

1.33±0.03

1.37±0.04

 

NKCC

0.92±0.28

1.77±0.44

1.27±0.07

1.97±0.43

1.56±0.12

2.05±0.21

 

1.19±0.15

1.08±0.33

0.93±0.16

 

1.02±0.20

 

1.31±0.15

1.50±0.12

 

1.28±0.02

 

1.51±0.11

1.18±0.08

1.46±0.05

1.10±0.08

0.96±0.00

  1. Larvae were reared at control PCO2 (C) and high PCO2 (CO2) at control temperature (10°C) or exposed to a heat shock for 5 h at 20°C. Arrow direction indicates significantly higher (upwards) or lower (downwards) gene expression between CO2 treatments at the same temperature or between temperatures within the same CO2 treatment. Black arrows: CO2 effect at the same temperature (10°C or 20°C). White arrows: heat shock effect. White/Black arrows in one direction indicate a combined effect of CO2 and heat shock.