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Table 2 Gene expression analysis: gene expression (quantities) in zoea and megalopa larvae of Hyas araneus at different time points in development classified according to their function in cellular stress/heat shock response

From: Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2

 

Cellular stress/heat shock response

 

Zoea I day 0

Zoea I day 15

Zoea II day 3

Zoea II day 15

Megalopa day 3

Gene

C

C

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

10°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

HSP 70_1

0.39±0.02

1.85±0.29

0.36±0.03

0.42±0.06

1.72±0.10

2.09±0.20

0.77±0.07

0.66±0.10

1.31±0.08

1.76±0.07

0.27±0.03

0.25±0.01

1.00±0.16

1.51±0.11

0.19±0.01

0.24±0.03

1.04±0.10

0.94±0.21

HSP 70_2

0.92±0.14

2.14±0.38

0.84±0.07

1.01±0.11

 

1.97±0.15

2.19±0.08

0.47±0.01

0.40±0.02

1.11±0.13

 

0.88±0.06

 

0.69±0.05

0.83±0.04

1.46±0.14

1.91±0.11

0.71±0.05

0.73±0.04

1.59±0.08

1.40±0.09

HSP 70_3

0.39±0.10

1.19±0.19

0.56±0.07

1.03±0.25

 

1.55±0.25

1.87±0.29

0.85±0.03

0.77±0.10

1.18±0.13

1.08±0.07

0.64±0.12

0.71±0.04

1.15±0.04

1.56±0.12

0.44±0.05

0.64±0.03

1.05±0.17

1.17±0.16

HSP 70_4

0.94±0.27

2.17±0.41

 

0.84±0.05

1.56±0.24

1.87±0.17

2.43±0.19

0.75±0.04

0.81±0.10

1.23±0.15

1.81±0.11

0.69±0.04

0.88±0.07

1.31±0.03

1.58±0.13

0.51±0.03

0.89±0.10

1.71±0.23

1.41±0.18

HSP 90

1.01±0.32

1.82±0.29

 

0.94±0.09

1.10±0.16

 

1.90±0.22

2.13±0.19

1.26±0.05

1.11±0.22

1.41±0.16

 

1.84±0.04

0.81±0.04

0.69±0.03

1.19±0.04

1.63±0.07

0.55±0.02

0.66±0.07

1.89±0.19

1.40±0.11

 

HSP 26

1.18±0.24

2.16±0.37

 

1.08±0.18

2.00±0.29

0.97±0.22

 

1,48±0.36

 

1.47±0.09

1.68±0.37

0.80±0.07

 

1.16±0.09

 

2.23±0.10

2.03±0.13

1.69±0.08

1.61±0.12

1.28±0.08

1.57±0.05

1.22±0.13

 

1.28±0.09

 

HSP 60

1.40±0.10

1.98±0.43

 

1.56±0.12

2.08±0.43

 

1.94±0.05

 

2.10±0.18

 

1.32±0.15

1.25±0.24

0.90±0.08

 

0.99±0.18

 

1.11±0.13

1.53±0.12

1.24±0.02

 

1.61±0.18

 

1.08±0.05

0.91±0.08

1.40±0.31

 

1.01±0.12

 
  1. Larvae were reared at control PCO2 (C) and high PCO2 (CO2) at control temperature (10°C) or exposed to a heat shock for 5 h at 20°C. Arrow direction indicates significantly higher (upwards) or lower (downwards) gene expression between CO2 treatments at the same temperature or between temperatures within the same CO2 treatment. Black arrows: CO2 effect at the same temperature (10°C or 20°C). White arrows: heat shock effect. White/Black arrows in one direction indicate a combined effect of CO2 and heat shock.