Figure 5

The brain, ciliary sensory cells, and glial repeats of the head or prostomium: whole-mount laser confocal microscopy images. A: Dorsal view of the prostomium with buccal tentacles. B: A semi-sagittal histological section cut as shown in A, showing a negative image of the hematoxylin and eosin stained section. Pseudocolors were used to enhance the position of line type cilia (light blue), axonal bundles and the brain (green), and secretory cells in the nuchal groove (yellow). Inset: granules stained with eosin. The arrowheads indicate the same position. C: A side view of the prostomium showing the distribution of acetylated alpha-tubulin (acTUBA) positive ciliary cells. The arrowheads indicate the line type cilia. The concanavalin A (conA, blue) and phalloidin (actin, red) cell membrane or muscle markers used for counterstaining. D, E: The sensory cell bodies and their axons coupled with dye (neurov: NeuroVue®Red) and repeated myelin-like glia stained with conA membrane marker (blue): a dorsal view of whole-mount prostomium and the enlargement. F-G: The sensory cells and the axonal projection patterns in the image series of conA, neurov, and merge. The numbers indicate the glial repeats and the arrowheads are positions filled with dye, and some labeled axons in the brain. I: Enlarged view showing the axons in the glial repeats. br, brain; bt, buccal tentacle; mo, mouth opening; pil, neuropil. Scale bar in A-C: 100 μm; D-H: 40 μm; I: 20 μm.