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Figure 5 | Frontiers in Zoology

Figure 5

From: Branchial NH4+-dependent acid–base transport mechanisms and energy metabolism of squid (Sepioteuthis lessoniana) affected by seawater acidification

Figure 5

Localization of acid–base transporters in gill epithelia of squid Sepioteuthis lessoniana . Immunohistochemical analyses demonstrated co-localization of acid–base transporters including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), V-type H+-ATPase (VHA) and Rhesus protein (RhP) in the ion-transporting epithelium of the squid gill. NKA is located in basolateral membranes whereas the NHE3 specific antibody shows positive immunoreactivity in apical membranes (A). VHA is located in basolateral membranes as well as pillar-cells spanning through the blood sinus (B). The RhP specific antibody shows positive immunoreactivity in apical membranes of the inner, ion-transporting branchial epithelium (C). High magnification image of a pilaster (pillar) cell showing positive VHA immunoreactivity. Dashed lines indicate the contour of the cell and the epithelial cells lining the blood sinus (D). Western blot analyses using gill homogenates, indicating specific immune-reactivity of the different antibodies with proteins in the predicted size range (indicated by arrows) (E). blood sinus (bs); inner epithelium (ie); outer epithelium (oe).

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