Figure 3

Extracellular acid–base status in Sepioteuthis lessoniana during acclimation to acidified conditions. Time course of in vivo changes in blood acid–base parameters including extracellular pH (pHe) (A), HCO₃⁻(B) and p CO₂(C) during 168 h exposure to control (pH 8.1) and acidified (pH 7.7 and pH 7.3) conditions. The exposure period was separated into a short-term (grey) and medium-term (white) acclimation period. Letters indicate significant differences between pH treatments (p > 0.05). Bars represent mean ± SE (n = 3 replicate tanks, with six biological replicates per time point and treatment).