Figure 2From: Branchial NH4+-dependent acid–base transport mechanisms and energy metabolism of squid (Sepioteuthis lessoniana) affected by seawater acidificationEffects of seawater acidification on metabolic rates and ammonia excretion. Routine metabolic rates were determined after 20 h (A) and 168 h (B) exposure to acidified conditions. NH4+ excretion rates determined after 20 h and 168 h exposure to different pH conditions are presented in (C) and (D), respectively. O:N ratios calculated from oxygen consumption and NH4+ excretion are given for short-term (E) and medium-term (F) exposure to different pH conditions. Letters indicate significant differences between pH treatments (p < 0.05). Bars represent mean ± SE (n = 3; with the average of 2 animals from each replicate).Back to article page