Marked changes in neuropeptide expression accompany broadcast spawnings in the gastropod Haliotis asinina

Table 1 Oligonucleotide primers for qPCR

Target gene	Primers	Tm
	(F = forward and R = reverse)
Has-APGWamide	F: TCTGAGGCTGGGAGATCAAAGG	68°C
	R: ACGTGTTTGGGAACATCCAGTAGG
Has-Whitnin	F: CACGCCAAGAGGACTTTGAGATGG	68°C
	R: GGGTATGGGATGAAGTGGTTGTGTG
Has-Myomodulin	F: ACGGTCGGACGAGAAAGTTGATGG	68°C
	R: TGATGGGTTTGTTGGGAGGGATG
Has-FMRFamide	F: TTCGGGAAGCGAGATTCTGGTG	68°C
	R: GGTGGATGTGAAACAGCGAACAGTC
Has-SLP	F: TCGTCCTCATCGCTCTCGTTGTT	69°C
	R: GGAGCCGTTGGAAATGCAGAAG
Has-MIP	F: CAAGCGGACGAGTGAACAAGG	67°C
	R: TTGCTCTTACAGCGTCTAGCATGG
Has-HGAP	F: CTGTGCATTCTCCTTGTCGTAGTCG	68°C
	R: GCTCATGTAGCCGAATGATTCTTCC
Reference genes
(Williams et al., 2009)
Has-Ubiquitin	F: TGGCAAGCAGTTGGAAGATGGT	57°C
	R: CAGTTGTACTTGGAGGCCAGGAT
Has-NACA	F: TGTCGCAAGCCAACGTTTCA	57°C
	R: GACAGCATGTTCAGCACTGGT
Has-DAu1506	F: AGATGCGTGTATGCTGGAGT	57°C
	R: TGGGTACATGCCAATGCT

ISSN: 1742-9994