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Figure 3 | Frontiers in Zoology

Figure 3

From: Marked changes in neuropeptide expression accompany broadcast spawnings in the gastropod Haliotis asinina

Figure 3

Characterisation of  Has-Whitnin  . (A) Nucleotide and predicted amino acid sequence of Has-Whitnin (nucleotide and amino acids are numbered on left). A predicted signal sequence is underlined. Predicted monobasic and dibasic basic cleavage sites are boxed. Predicted peptides cleaved from the precursor are shaded. Putative disulphide bonded cysteines within the predicted ERYM peptide are circled. Nucleotides 246–269 and 399–423, in bold, correspond to the Has-Whitnin qPCR primers. (B) Multiple sequence alignment of H. asinina Whitnin prepropeptide with molluscan homologues. The 4-level shading indicates conservation, as detailed in Nicholas et al. (1997) [33]. Haliotis H. asinina GenBank:JN606063; Lottia Lottia gigantea[34]; Lymnaea Lymnaea stagnalis ([GenBank:AAF36485]; [42]); Aplysia A. californica ([GenBank:AAV84472]; [41]). (C) Schematic representation of H. asinina anterior ganglia showing regions analysed by MALDI-TOF-MS. MALDI-TOF-MS detection of predicted Has-Whitnin gene products in regions of H. asinina anterior ganglia. Asterisks indicate that masses consistent with both disulphide bonded and non-disulphide bonded versions of the predicted ERYM peptide were found in LG1 and RG2. Peaks shown represent LPADEamide (m/z 541.7), SPTR (m/z 1656.6), and ERYM with disulphide bonded cysteines (m/z 2699.9) (right). Signal, signal sequence; LG1, left cerebral ganglia region 1; LG2, left cerebral ganglia 2; RG1, right cerebral ganglia 1; RG2, right cerebral ganglia 2; CC, cerebral commissure; PGM, pleuropedal ganglia middle; amide, amidation; m/z, mass to charge ratio.

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