Metabolic shifts in the Antarctic fish Notothenia rossii in response to rising temperature and P CO2

Table 4 Intracellular and extracellular pH (pH _{i/ e} ) and acid–base parameters in white muscle/ liver tissue homogenates and blood, respectively, from N. rossii . Control: 1°C, 0.04 kPa CO ₂ ; warm normocapnia: 7°C, 0.04 kPa CO ₂ ; cold hypercapnia: 1°C, 0.2 kPa CO ₂ ; warm hypercapnia: 7°C, 0.2 kPa CO ₂

Acclimation		White muscle homogenate				Liver homogenate				Blood acid–base parameters
T (°C)	P CO₂(kPa)	N	P CO₂(kPa)	[HCO₃^-] (mmol*l^-1)	pHi	N	P CO₂(kPa)	[HCO₃^-](mmol*l^-1)	pHi	N	P CO₂(kPa)	[HCO₃^-] (mmol*l^-1)	pHe
1	0.04	3	0.640±0.126	3.994±0.410	7.325±0.065	3	2.555±0.270	9.213±0.379	7.080±0.027	9	1.18±0.19	8.05±0.56	7.438±0.055
7	0.04	5	1.071±0.123	5.294±0.335	7.242±0.037	1	3.616	8.020	6.890	9	1.16±0.05	6.31±0.28^a,b	7.315±0.045^b
1	0.2	5	0.936±0.117	6.724±0.664^a	7.383±0.027	5	3.031±0.484	10.107±1.197	7.053±0.066	5	1.46±0.09	11.28±0.32^a,b,c	7.508±0.028
7	0.2	10	1.296±0.167	6.849±0.373^a	7.290±0.038	7	4.495±0.521	10.347±0.724	6.920±0.039	10	1.41±0.11	10.08±0.57^a	7.507±0.037

Data are presented as means ± SEM. Superscript letters indicate following significant differences: a – sign. different to control conditions, b – sign. different to temperature & CO₂ acclimation. c –sign. different to temperature acclimation (t-test, ANOVA, p≤ 0.05).

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