Figure 4
Whole mounts of adult specimens stained with antibodies against FMRFamide (green: A, B, C, F, F’, F”), serotonin (monoclonal; magenta: A, B, C, F, F’, F”), and fluorophore-tagged phalloidin (blue; D, E). A. Dorsal side of entire specimen. B. Anterior end. Arrows point to lateral varicosities of neurites that probably connect the lobes with receptor cells in the epidermis. C. Detail of B. D. Anterior half of a specimen showing RFL immunoreactive parts of the brain and neurites surrounding the dorso-ventral muscles of the ventral groove. E. Musculature of copulatory organs innervated by RFL immunoreactive neurites. F. Dorsal projection of brain. F’. Central projection of brain. F”. Ventral projection of brain. Abbreviations: 1 dorsal neurite bundle; 2 lateral neurite bundle; 3 ventral neurite bundle; bn bursal nozzle; cop male copulatory organ; ds digestive syncytium; fr frontal ring; pc dorsal posterior commissure. Scale bars: A 100 μm; B 50 μm; C 10 μm; D 50 μm; E 50 μm; F-F” 25 μm.