Figure 4

Transcriptional activation of HDACs after the administration of HDAC inhibitors prior to septic injury. The transcription of (A) histone deacetylase 8, (B) histone deacetylase 8 isoform 2, (C) histone deacetylase complex and (D) histone deacetylase complex subunit sap 18 was measured by quantitative real time RT-PCR following the injection of HDAC inhibitors (1:1 SAHA (1 mg/ml) and sodium butyrate (20 mg/ml)) prior to injury and hemolymph loss, relative to the expression levels in control larvae treated with 1% DMSO. Values were normalized against the 18S rRNA housekeeping gene and represent means of three independent measurements ± standard deviations (*, p < 0.05).