Table 1 Specimens investigated with methods applied in addition to LM serial section examination
Specimen number (spm) | Specimen length | Embedding medium | Section thickness | TEM | Number of LM sections used for subsequent TEM sectioning | Computerized 3D analysis (Amira software) |
|---|---|---|---|---|---|---|
3 | 415 μm | Spurr’s lvr | 1.5 μm | no | - | SR, total specimen |
3a | 504 μm | Spurr’s lvr | 1.5 μm | no | - | SR, metanephridial region |
4 | 513 μm | Spurr’s lvr | 1.5 μm | yes | 10 | SR, metanephridial region |
4a | 522 μm | Agar lvr | 1.5 μm | yes | 2 | aligned 3D stack |
4b | 558 μm | Agar lvr | 1.5 μm |  | - | - |
5 | 579 μm | Spurr’s lvr | 1.5 μm | no | - | SR, total specimen |
6 | 580 μm | Spurr’s lvr | 1.5 μm | yes | 12 | SR, metanephridial region |
7 | 604.5 μm | Spurr’s lvr | 1.5 μm | yes | 17 | SR, total specimen |
7a | 594 μm | Spurr’s lvr | 1.0 μm | no | - | aligned 3D stack |
8 | 621 μm | Spurr’s lvr | 1.5 μm | yes | 14 | SR, total specimen |
9 | 652.5 μm | Spurr’s lvr | 1.5 μm | yes | 7 | aligned 3D stack |
10 | 753 μm | Spurr’s lvr | 1.5 μm | yes | 17 | SR, total specimen |
11 | ~800 μm | Spurr’s lvr | 1.5 μm / 1.0 μm | yes | - | - |
|  |  |  | 50–70 nm |  |  |  |
12 | 852 μm | Spurr’s lvr | 1.5 μm | no | - | SR, total specimen |
13 | 886.5 μm | Spurr’s lvr | 1.5 μm | yes | 12 | SR, metanephridial region |
14 | 1159.5 μm | Spurr’s lvr | 1.5 μm | no | - | SR, total specimen |
15 | 1465.5 μm | Agar lvr | 1.5 μm | yes | 2 | - |
16 | 1821 μm | Agar lvr | 1.5 μm | no | - | - |