eagle mRNA localisation in Delta RNAi ovarioles. Wildtype (A) and Delta RNAi (B) ovarioles stained for tubulin (green) and eagle mRNA (red). (A) In Wildtype ovarioles, eg mRNA becomes restricted to the anterior part of the oocyte (arrowheads). (B) Four days after dsRNA injection the Delta phenotype is obvious, as the stalk is absent and egg chambers are directly attached to each other. While, in older egg-chambers transcripts become localized to the anterior pole of the oocyte (white arrowheads), in early egg-chambers mRNA localization occurs premature and perpendicular to the apparent anterior-posterior axis of the egg-chamber (red arrowheads, B1 close up of the ovariole in B).