Delta RNAi affects encapsulation. Wildtype (A) and Delta (E) RNAi ovarioles stained for f-actin with Phalloidin (red), DNA with Hoechst (blue). (C, E) phalloidin channel only. Roman numerals refer to egg-chambers in corresponding stages of oogenesis. (B-D) Close up of the wildtype ovariole shown in (A) (D, schematic representation). During encapsulation, pro-oocytes (I) first come in contact with lateral pre-follicular cells, which successively engulf the oocytes to form an egg-chamber (II). As these early follicles develop, they become aligned in a single row (III). (E,F) In Delta RNAi, encapsulation is incomplete (compare stage III egg-chambers in D to F). Pro-oocytes are not separated from each other, resulting in egg chambers comprising of multiple oocytes.