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Table 2 Insoluble chromogenic substrates used to test for enzyme activity and the specific type of enzymes measured

From: Patterns of functional enzyme activity in fungus farming ambrosia beetles

Substrate

Enzyme

Cellulose

 

AZCL-HE-Cellulose

cellulase (endo-β-1,4-glucanase)

AZCL-Barley β-Glucan

cellulase (endo-β-1,3(4)-glucanase)

AZCL-Xyloglucan

endo-β-1,4-xyloglucanase

Hemicellulose

 

AZCL-Xylan

endo-β-1,4-xylanase

AZCL-Arabinoxylan

endo-β-1,4-xylanase

AZCL-Dextran

endo-α-1,6-dextranase

Pectin

 

AZCL-Debranched Arabinan

endo-α-1,5-arabinase

AZCL-Rhamnogalacturonan

rhamnogalacturonanase

AZCL-Galactomannan

endo-β-1,4-mannanase

AZCL-Galactan

endo-β-1,4-galactanase

Protein

 

AZCL-Casein

endo-protease

AZCL-Collagen

endo-protease

Starch

 

AZCL-Amylose

α-amylase

  1. AZCL = Azurine cross-linked polysaccharides (Megazyme©, Bray, Ireland).