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Table 4 Dilution assay of sex-inducing activity in the M0 + M10 fraction of Dugesia ryukyuensis

From: Sex-inducing effect of a hydrophilic fraction on reproductive switching in the planarian Dugesia ryukyuensis (Seriata, Tricladida)

Dilution series

Number of test worms that developed a pair of ovaries (%)

Number of test worms that developed a copulatory apparatus (%)

1

45/50 (90.0 ± 6.0)

38/50 (76.0 ± 16.0)

1/2

40/50 (80.0 ± 4.0)

14/50 (28.0 ± 4.0)

1/5

35/49 (71.5 ± 3.5)

0/49 (0.0 ± 0.0)

1/10

33/50 (62.0 ± 2.0)

0/50 (0.0 ± 0.0)

1/25

3/50 (6.0 ± 6.0)

0/50 (0.0 ± 0.0)

1/125

0/48 (0.0 ± 0.0)

0/48 (0.0 ± 0.0)

1/625

0/50 (0.0 ± 0.0)

0/50 (0.0 ± 0.0)

  1. To prepare test food, each dilution sample was mixed with 150 μl of chicken liver homogenate and then freeze-dried. Twenty-five test worms were fed for 4 weeks. Means and standard errors of duplicated experiments are shown.
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Frontiers in Zoology

ISSN: 1742-9994