Figure 8

Series of conventional light microscopy images of P. dumerilii , 24-36 hpf. For each time point an overview (left) and the larval eye at higher magnification (right) is shown. All images except O and P are apical view of larvae, dorsal side up. O: Ventro-posterior view of the larva at 36 hpf, apical side left. P: Anterior-ventral view of a larva at 36 hpf, apical side left. A, C, E, G, I, K, M, O: In all larva the 4 macromeres (mm) in the center of the larva is visible. Each macromere contains one lipid droplet (ld). The two ventral lipid droplets are smaller than the dorsal ones. Around the prototroch additional pigment (pi) is visible in some larvae (I, K, M, O). B, D, F, H, J, L, N, P: The pigmentation of the larval eyes (le) becomes more intense over time. Scale bar in all images 20 μm. Further abbreviations see abbreviations list. In this and all other figure plates, fresh samples were used at every time point. Each specimen was collected from 18°C ± 0.1°C source immediately before imaging.