Figure 5

BrdU detection in pulse and chase and in continuous labeling experiments. A, B. Whole-mount detection of BrdU in a tetrathyridium after a 24 hour pulse followed by a 48 hour chase. C. Whole-mount detection of BrdU in early segments of an adult worm after a 24 hour pulse followed by a 48 hour chase. D. Sagittal section of tetrathyridium after a 4 hour pulse followed by a 68 hour chase. E. Section of tetrathyridium with an amputated scolex after 72 hours of continuous labeling with BrdU. cp, cortical parenchyma; iml, inner muscle layer; mp, medullary parenchyma; st, sub-tegument; t, tegument. Note that the tegument was removed in whole-mount specimens. Bars represent 100 μm in A, 50 μm in B and C, and 20 μm in D and E.