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Figure 6 | Frontiers in Zoology

Figure 6

From: Embryonic and post-embryonic development of the polyclad flatworm Maritigrella crozieri; implications for the evolution of spiralian life history traits

Figure 6

CLSM micrographs of the nervous system in two-day post-hatching Maritigrella crozieri. (phalloidin-white, acTub-green, 5HT-magenta, FMRFamide-blue [except in C where 5HT and FMRFamide-magenta]). (A) Mid optical sections of triple labeled larva (ventral view), showing neural components; commissural cell bodies [ccb], dorso-lateral connective [dlc], pharyngeal nerve ring [phnr], intra-epidermal cells [iec], epidermal nerve plexi [enp], apical plate [ap] and apical cilium [ac], in relation to the apical complex muscles (ac) and posterior primary circular muscle (ppcm). (B) FMRFamide expression in the apical plexus (apl), dorso-lateral coonective, pharyngeal nerve ring and supra-oral nerve ring (arrowhead) Ventral view. Rhabdites (rb). (C) Dorso-lateral view showing the neural activity in the apical plexus, the cerebral commissure (cc) and commissural cell bodies, and the spatial relationships between the intra-epidermal cells, epidermal nerve plexi, pharyngeal nerve ring, protonephridia (pnp), and intestine (in). (D & E) Ventral and lateral views of the nerve and muscle orthogons showing the ventro-lateral (vlc) and dorso-lateral connectives, the innervation of the sub-oral plate (caudal perikarya (cp) and caudal cilia perikaryon (ccip) on the dorso-lateral connective and ventral perikarya (vp) on the medial nerve), and the intra-epidermal 5HT expression at the apical plate and the ciliary band (cb). (F) Dorsal view showing the spatial relations of the protonephridia (pnp) and dorso-lateral connectives, the pharyngeal nerve ring and the pharynx (ph) and intestine, and the intra-epidermal cells and the neuropile and commissural cell bodies. (G) Right lateral view of head, 5HT expression in epidermal nerve plexus external to the apical complex of body wall muscles and acTub IR in intra-epidermal cells. (H) Neuromusculature of left lateral lobe (lll) showing nerves external to body wall muscles. (I) Ventral view of FMRFamide expression in the pharyngeal nervous system shows connections between the pharyngeal nerve ring and the dorso-lateral connectives (arrowheads) and cerebral commisure (double arrowheads). Scale bars 50 μm.

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