Figure 1
Confocal laser scanning micrographs (CLSM) of the blastula and gastrula stages of Maritigrella crozieri. (F-actin labeled with phalloidin 564 [red], nucleic acids stained with Sytox Green [green]). (A-D) - animal views of cleavage cycles 1-4; showing the first quartet micromeres (arrowheads in C) and second quartet micromeres (arrowheads in D). (E) Animal view showing the third quartet micromeres (arrowheads), vegetal view showing large fourth quartet micromeres (4a-d, double arrowheads) and mini-macromeres (mm)(4A-D). (F) Lateral view shows irregular double layer of dividing 1st to 3rd quartet micromeres at the animal pole over the 4a-d quartet. (G) Animal view of micromere cap and vegetal view of 4d division. (H-J) Animal, lateral and vegetal views respectively of gastrulation by epiboly; micromere cap migrates vegetally enveloping the fourth quartet micromeres and macromeres, which remain visible until the ectoderm invaginates at the lower pole to form the stomodeum. Scale bars 100 μm, hpo = hours post oviposition, dt = development time.