Bacterial feeding induces changes in immune-related gene expression and has trans-generational impacts in the cabbage looper (Trichoplusia ni)

Table 3 RT-qPCR results for adults fed on different diets for genes identified by GeneFishing.

Gene	Genders pooled	Females	ct value	Males	ct value
*Defense*
cathepsin L-like protease	1.01 ± 0.100	1.02 ± 0.046	18.23	2.16 ± 0.158	17.46
cytochrome c oxidase polypeptide Vb	1.41 ± 0.010
mitochondrial cytochrome c oxidase subunit VIa	1.29 ± 0.045
Mn superoxide dismutase	1.27 ± 0.050	-1.01 ± 0.036	19.87	1.89 ± 0.097	19.57
lectin 4 C-type lectin	1.47 ± 0.005
Glutathion S-transferase 1	1.04 ± 0.310	1.59 ± 0.258	30.63	1.54 ± 0.236	30.38
ubiquinol-cytochrome c reductase	1.01 ± 0.070
*Development*
trypsin-like serine protease	-1.19 ± 0.040
hypothetical protein AaeL_AAEL012245, contains chitin binding domain	-2.91 ± 0.240	-1.43 ± 0.050	27.8	1.55 ± 0.015	28.75
Perithrophin A	-1.08 ± 0.115
myosin I	-1.18 ± 0.115
titin1	-1.42 ± 0.090	-1.06 ± 0.236	19.52	1.17 ± 0.356	18.43
*Digestion*
tyrosine-protein kinase	-1.31 ± 0.000
*DNA related*
C14orf124 protein	1.07 ± 0.060
zinc finger protein	-1.18 ± 0.070
*Metabolism*
alcohol dehydrogenase (acceptor)	2.20 ± 0.005	1.44 ± 0.285	28.36	2.08 ± 0.030	30.64
protease inhibitor 1	1.52 ± 0.010	1.04 ± 0.175	19.56	1.17 ± 0.305	16.42
Serine/threonine-protein kinase polo	-1.20 ± 0.045
short-chain dehydrogenase	1.15 ± 0.175
protein disulfide isomerase	1.16 ± 0.020
*Signaling*
putative G-protein coupled receptor	-1.64 ± 0.110	1.05 ± 0.245	27.92	-1.41 ± 0.150	30.15

Results are shown for animals grown on bacterial diet, where bacteria-free diet treatments were set to 1 (values are mean ± SD).

ISSN: 1742-9994