Table 2 RT-qPCR results for 9 day old larval midguts and non-midgut tissue (restbody) for genes identified by GeneFishing.
Gene | Midgut | ct value (Bac) | Restbody | ct value (Bac) |
|---|---|---|---|---|
Defense | Â | Â | Â | Â |
cathepsin L-like protease | 1.77 ± 0.018 | 19.99 | 1.17 ± 0.034 | 19.22 |
cytochrome c oxidase polypeptide Vb | 1.07 ± 0.074 | 17.81 | -1.08 ± 0.094 | 18.61 |
mitochondrial cytochrome c oxidase subunit VIa | -1.02 ± 0.115 | 17.93 | -1.15 ± 0.004 | 19.3 |
Mn superoxide dismutase | -1.03 ± 0.026 | 20.18 | -1.75 ± 0.030 | 20.38 |
lectin 4 C-type lectin | -3.49 ± 0.048 | 25.53 | -1.96 ± 0.129 | 21.9 |
Glutathion S-transferase 1 | 1.80 ± 0.037 | 20.35 | -9.17 ± 0.058 | 27.94 |
ubiquinol-cytochrome c reductase | -1.16 ± 0.005 | 19.27 | -1.10 ± 0.014 | 19.74 |
Development | Â | Â | Â | Â |
trypsin-like serine protease | -1.35 ± 0.212 | 32.96 | 2.02 ± 0.037 | 23.89 |
hypothetical protein AaeL_AAEL012245, contains chitin binding domain | -1.29 ± 0.210 | 31.98 | -1.42 ± 0.163 | 31.89 |
Perithrophin A | -78.25 ± 0.067 | 29.15 | -1.28 ± 0.148 | 28.17 |
myosin I | -1.27 ± 0.014 | 22.73 | -1.72 ± 0.035 | 22.47 |
titin1 | -2.32 ± 0.018 | 20.1 | 1.29 ± 0.028 | 16.64 |
Digestion | Â | Â | Â | Â |
tyrosine-protein kinase | -1.40 ± 0.134 | 25 | 3.96 ± 0.007 | 28.89 |
DNA related | Â | Â | Â | Â |
C14orf124 protein | -1.03 ± 0.023 | 22.48 | -1.15 ± 0.030 | 21.59 |
zinc finger protein | -1.05 ± 0.065 | 21.13 | -1.08 ± 0.267 | 21.97 |
Metabolism | Â | Â | Â | Â |
alcohol dehydrogenase (acceptor) | -2.05 ± 0.939 | 19.8 | 2.25 ± 0.062 | 22.76 |
protease inhibitor 1 | -2.45 ± 0.325 | 29.81 | -1.18 ± 0.131 | 19.04 |
Serine/threonine-protein kinase polo | -1.45 ± 0.097 | 31.06 | -1.17 ± 0.048 | 26.2 |
short-chain dehydrogenase | -1.20 ± 0.018 | 21.09 | 6.12 ± 0.117 | 26.29 |
protein disulfide isomerase | -1.09 ± 0.078 | 17.63 | 1.25 ± 0.074 | 17.66 |
Signaling | Â | Â | Â | Â |
putative G-protein coupled receptor | -1.40 ± 0.25 | 19.31 | 9.48 ± 0.129 | 25.49 |