Base composition, selection, and phylogenetic significance of indels in the recombination activating gene-1 in vertebrates

Table 7 Comparative analysis for each vertebrate group of the 5' and 3'-ends of Rag1

	Variables	t	df	p-value
Amphibia	Tamura-Nei nucleotide distances	17.4	3	0.0004137*
	Number of variable sites	3.4447	3	0.04110*
Aves	Tamura-Nei nucleotide distances	5.7446	3	0.01048*
	Number of variable sites	3.1192	3	0.05251
Crocodylia	Tamura-Nei nucleotide distances	-	-	-
	Number of variable sites	5.1962	3	0.01385*
Lepidosauria	Tamura-Nei nucleotide distances	4.8414	3	0.01681*
	Number of variable sites	3.1114	3	0.05283
Mammalia	Tamura-Nei nucleotide distances	6.6541	3	0.006918*
	Number of variable sites	2.9613	3	0.05948
Testudines	Tamura-Nei nucleotide distances	9	3	0.002896
	Number of variable sites	2.5062	3	0.08723

Comparative analysis within each vertebrate lineage of the two parts of Rag1, 5' and 3'-ends. For each vertebrate lineage average Tamura-Nei nucleotide distances, and average number of variable nucleotide sites within group have been used for the analysis. Tamura-Nei nucleotide distances cannot be compared for Crocodylia as data are essentially constant between the two parts of the gene. Number of sequences used for each monophyletic vertebrate lineage: Amphibia = 7; Aves = 119; Crocodylia = 13; Lepidosauria = 80; Mammalia = 37; Testudines = 24. "df" indicates the degree of freedom. * indicates the significant p-value (p < 0.05).

ISSN: 1742-9994