Figure 1

Circular Neighbour-Joining tree for unique haplotypes of Trichoptera analyzed in this study. A total of 293 haplotypes represent 1500 COI sequences and 68 morphospecies of Trichoptera. Terminal branches of species with intraspecific divergence greater than 2% are highlighted in red. Species groups with subtle diagnostic characters or females/immatures that are difficult to identify morphologically are highlighted in green blocks. Members of these groups can easily be confused or neglected in routine morphological sorting, but are readily detected via barcoding. Red and pink bars in the outer circles represent the maximum intraspecific divergence and minimum distance to nearest neighbour, respectively, of the corresponding species shown in the circular tree. The heights of the two distance bars are proportional to the distance values (see Table S3 in Additional file 3). Two possible cryptic caddisfly species, Limnephilus sansoni and Cheumatopsyche campyla (Ross), are highlighted in yellow blocks.