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Table 2 Diet of Myodes rufocanus

From: Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures

season method

summer dna (N = 18)

Mic (N = 16)

autumn dna (N = 12)

mic (N = 11)

Arctous alpinus

.002 (± .01)

NA

0

NA

Avenella flexuosa

.01 (± .02)

.01 (± .03)

0

0

Bistorta vivipara

.05 (± .11)

0

.01 (± .05)

0

Chamaepericlymenum suecica

.09 (± .17)

.004 (± .01)

.003 (± .01)

0

Deschampsia cespitosa

.01 (± .06)

0

0

.01 (± .02)

Empetrum nigrum hermafroditum

.12 (± .18)

.01 (± .03)

.05 (± .07)

0

Oxycoccos microcarpus

0

NA

.001 (± .004)

NA

Oxyria digyna

0

.003 (± .01)

0

0

Pedicularis lapponica

0

.004 (± .01)

0

0

Phleum alpinum

.04 (± .04)

0

.09 (± .06)

0

Pyrola minor

.0003 (± .001)

NA

0

NA

Rumex acetosa

.08 (± .12)

.004 (± .013)

.05(± .08)

0

Salix herbaceae

0

.01 (± .03)

0

0

Solidago virgaurea

.01 (± .04)

0

0

0

Trientalis europaea

.01 (± .02)

NA

.003 (± .01)

NA

Trollius europaeus

.002 (± .01)

NA

0

NA

Vaccinium myrtillus

.001 (± .003)

0

.01 (± .01)

.04 (± .05)

Vaccinium uliginosum microphyllum

.05 (± .06)

.02 (± .02)

.19 (± .31)

.01 (± .03)

Vaccinium vitis-idaea

.01 (± .03)

.01 (± .02)

0

.00 (± .01)

Viola biflora

.01 (± .05)

NA

0

NA

genus

    

Arctous

.002 (± .01)

NA

0

NA

Avenella

.01 (± .02)

.01 (± .03)

0

0

Betula

.01 (± .02)

NA

.04 (± .14)

NA

Bistorta

.05 (± .11)

0

.01 (± .05)

0

Chamaepericlymenum

.09 (± .17)

.004 (± .01)

.003 (± .01)

0

Deschampsia

.01 (± .06)

0

0

.01 (± .02)

Empetrum

.12 (± .18)

.01 (± .03)

.05 (± .07)

0

Epilobium

.01 (± .03)

NA

0

NA

Festuca

0

.01 (± .02)

0

0

Geranium

.002 (± .01)

NA

.04 (± .12)

NA

Hierachium

0

0

0

.004 (± .01)

Oxycoccos

0

NA

.001 (± .004)

NA

Oxyria

0

.003 (± .01)

0

0

Pedicularis

0

.004 (± .01)

0

0

Phleum

.04 (± .04)

0

.09 (± .06)

0

Pyrola

.0003 (± .001)

NA

0

NA

Ranunculus

.01 (± .05)

NA

.02 (± .08)

NA

Rumex

.08 (± .12)

.004 (± .013)

.05(± .08)

0

Salix

.13 (± .24)

.03 (± .05)

.02 (± .04)

.01 (± .04)

Solidago

0

.01 (± .04)

0

0

Trientalis

.01 (± .02)

NA

.003 (± .01)

NA

Trollius

.002 (± .01)

NA

0

NA

Vaccinium

.22 (± .16)

.19 (± .13)

.49 (± .33)

.19 (± .16)

Viola

.01 (± .05)

.002 (± .01)

0

0

Equisetum

.06 (± .22)

.11 (± .24)

.005 (± .02)

.02 (± .05)

Gymnocarpium

.002 (± .01)

NA

0

NA

family

    

Apiaceae

0

NA

.003 (± .01)

NA

Asteraceae

.03 (± .09)

.01 (± .04)

.03 (± .07)

.004 (± .01)

Betulaceae

.01 (± .02)

NA

.04 (± .14)

NA

Caryophyllaceae

0

.001 (± .01)

0

.03 (± .05)

Cornaceae

.09 (± .17)

.004 (± .01)

.003 (± .01)

0

Cyperaceae

.004 (± .01)

.001 (± .01)

0

.001 (± .01)

Ericaceae

.42 (± .34)

.20 (± .13)

.61 (± .32)

.19 (± .16)

Geraniaceae

.002 (± .008)

.001 (± .005)

.04 (± .12)

.03 (± .05)

Onagraceae

.01 (± .03)

NA

0

NA

Orobanchaceae

0

.004 (± .01)

0

0

Poaceae

.07 (± .06)

.03 (± .08)

.14 (± .13)

.04 (± .12)

Polygonaceae

.14 (± .20)

.03 (± .04)

.07 (± .09)

.01 (± .02)

Primulaceae

.01 (± .02)

NA

.003 (± .01)

NA

Pyrolaceae

.0003 (± .001)

NA

0

NA

Ranunculaceae

.01 (± .06)

NA

.03 (± .09)

NA

Salicaceae

.13 (± .24)

.03 (± .05)

.02 (± .04)

.01 (± .04)

Scrophulariaceae

0

.01 (± .02)

0

0

Violaceae

.01 (± .05)

.003 (± .01)

0

0

Equisetaceae

.06 (± .22)

.11 (± .24)

.005 (± .02)

.02 (± .05)

Polypodiaceae

.002 (± .01)

NA

0

NA

higher taxonomic levels

    

Eudicots

.86 (± .23)

.80 (± .28)

.86 (± .12)

.63 (± .15)

Monocots

.07 (± .07)

.04 (± .11)

.14 (± .13)

.04 (± .12)

Monilophytes

.06 (± .22)

.11 (± .24)

.005 (± .02)

.02 (± .05)

Euphyllophytes

.004 (± .02)

.03 (± .05)

0

.22 (± .11)

Bryophytes

NA

.01 (± .04)

NA

.01 (± .01)

Fungi

NA

.003 (± .01)

NA

.09 (± .13)

  1. Proportion of identified food items in the diet of Myodes rufocanus using microhistological (mic) and DNA-barcoding (dna) methods, summer and autumn, (mean ± standard deviation). At each taxonomic level, also the proportions from lower levels are included, except for Euphyllophytes where only the items which could not be assigned to a lower taxa are included. NA = Food item not identifiable with the method in question.