Skip to main content

Advertisement

Table 1 Diet of Microtus oeconomus

From: Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures

season method summer dna (N = 14) mic (N = 13) autumn dna (N = 4) mic (N = 4)
Anthoxanthum nipponicum .01 (± .02) .005 (± .02) .03 (± .03) .005 (± .01)
Avenella flexuosa .01 (± .01) .01 (± .04) .04 (± .06) .02 (± .03)
Bistorta vivipara .03 (± .10) .005 (± .02) .06 (± .08) 0
Chamaepericlymenum suecica 0 0 .01 (± .01) 0
Cirsium heterophyllum .01 (± .03) .01 (± .02) 0 0
Deschampsia cespitosa .02 (± .03) .02 (± .04) 0 .01 (± .01)
Oxyria digyna 0 .01 (± .02) 0 0
Phleum alpinum .01 (± .02) 0 .04 (± .04) 0
Poa alpina 0 NA .005 (± .01) NA
Rumex acetosa .37 (± .25) .002 (± .01) .18 (± .18) 0
Salix herbaceae 0 .001 (± .01) 0 .005 (± .01)
Solidago virgaurea 0 .002 (± .01) 0 .005 (± .01)
Stellaria nemorum .003 (± .01) NA 0 NA
Trientalis europaea .07 (± .23) NA .07 (± .15) NA
Trollius europeus 0 NA .02 (± .04) NA
Viola biflora .02 (± .04) NA .01 (± .02) NA
genus     
Anthoxanthum .01 (± .02) .005 (± .02) .03 (± .03) .005 (± .01)
Avenella .01 (± .01) .01 (± .04) .04 (± .06) .02 (± .03)
Bistorta .03 (± .10) .005 (± .02) .06 (± .08) 0
Calamagrostis .05 (± .14) NA .02 (± .02) NA
Cerastium .04 (± .08) NA 0 NA
Chamaepericlymenum 0 0 .01 (± .01) 0
Cirsium .01 (± .03) .01 (± .02) 0 0
Deschampsia .02 (± .03) .02 (± .04) 0 .01 (± .01)
Festuca .01 (± .02) .03 (± .07) .03 (± .04) .01 (± .01)
Geranium 0 NA .01 (± .03) NA
Oxyria 0 .01 (± .02) 0 0
Poa 0 .01 (± .01) .005 (± .01) 0
Phleum .01 (± .02) 0 .04 (± .04) 0
Ranunculus .10 (± .12) NA .08 (± .06) NA
Rumex .37 (± .25) .002 (± .01) .18 (± .18) 0
Salix .16 (± .15) .01 (± .03) .08 (± .12) .02 (± .03)
Solidago 0 .002 (± .01) 0 .005 (± .01)
Stellaria .003 (± .01) NA 0 NA
Trientalis .07 (± .23) NA .07 (± .15) NA
Trollius 0 NA .02 (± .04) NA
Vaccinium 0 0 .10 (± .13) .005 (± .01)
Viola .03 (± .06) 0 .01 (± .02) 0
Equisetum .01 (± .02) .15 (± .23) .01 (± .02) .01 (± .01)
family     
Apiaceae .01 (± .04) NA 0 NA
Asteraceae .02 (± .04) .01 (± .03) .07 (± .13) .01 (± .01)
Caryophyllaceae .05 (± .10) .05 (± .12) 0 .06 (± .08)
Cornaceae 0 0 .01 (± .01) 0
Cyperaceae 0 .01 (± .03) 0 .02 (± .04)
Geraniaceae 0 NA .01 (± .03) NA
Ericaceae 0 0 .12 (± .16) .01 (± .01)
Poaceae .11 (± .13) .33 (± .35) .21 (± .20) .31 (± .23)
Polygonaceae .43 (± .29) .16 (± .17) .26 (± .13) .04 (± .08)
Primulaceae .07 (± .23) NA .07 (± .15) NA
Ranunculaceae .11 (± .13) NA .10 (± .10) NA
Rosaceae 0 .002 (± .01) .03 (± .06) 0
Salicaceae .16 (± .15) .01 (± .03) .08 (± .12) .02 (± .03)
Scrophulariaceae .001 (± .01) 0 0 0
Violaceae .03 (± .06) 0 .01 (± .02) 0
Equisetaceae .01 (± .02) .15 (± .23) .01 (± .02) .01 (± .01)
higher taxonomic levels     
Eudicots .88 (± .14) .47 (± .37) .78 (± .19) .54 (± .23)
Monocots .11 (± .13) .35 (± .36) .21 (± .19) .35 (± .29)
Monilophytes .01 (± .02) .15 (± .23) .01 (± .02) .01 (± .01)
Euphyllophytes 0 .02 (± .03) 0 .09 (± .13)
Bryophytes NA .01 (± .02) NA .01 (± .02)
Fungi NA 0 NA .01 (± .03)
  1. Proportion of identified food items in the diet of Microtus oeconomus using microhistological (mic) and DNA barcoding (dna) methods, summer and autumn, (mean ± standard deviation). At each taxonomic level, also the proportions from lower levels are included, except for Euphyllophytes where only the items which could not be assigned to a lower taxa are included. NA = Food item not identifiable with the method in question.