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Table 2 Minimum cumulative errors (MCE). Minimum cumulative errors relative to standard threshold (MCEST) and optimum threshold (MCEOT) for different markers and different data handling.

From: Integrated taxonomy: traditional approach and DNA barcoding for the identification of filarioid worms and related parasites (Nematoda)

marker

alignment

distance calculation

gap treatment*

ST

MCEST (%)

OT

MCEOT (%)

coxI

MUSCLE

TREECON

TA

4.6

0.3

4.5

0.3

coxI

MUSCLE

TREECON

NTA

4.6

0.3

4.5

0.3

coxI

MUSCLE

MEGA

PD

4.6

0.3

3.9

0.3

coxI

MUSCLE

MEGA

CD

4.2

0.3

4.5

0.3

coxI

Clustal

TREECON

TA

4.6

0.3

4.5

0.3

coxI

Clustal

TREECON

NTA

4.6

0.3

4.5

0.3

coxI

Clustal

MEGA

PD

4.6

0.3

3.9

0.3

coxI

Clustal

MEGA

CD

4.2

0.3

4.5

0.3

12S rDNA

MUSCLE

TREECON

TA

34.7

99.9

9.0

0.4

12S rDNA

MUSCLE

TREECON

NTA

22.0

88.7

6.4

0.3

12S rDNA

MUSCLE

MEGA

PD

22.0

89.9

6.7

0.3

12S rDNA

MUSCLE

MEGA

CD

12.0

54.7

5.8

1.9

12S rDNA

Clustal

TREECON

TA

22.0

33.5

7.2

0.4

12S rDNA

Clustal

TREECON

NTA

14.3

14.5

5.8

0.4

12S rDNA

Clustal

MEGA

PD

14.3

14.8

5.8

0.4

12S rDNA

Clustal

MEGA

CD

8.1

16.2

4.4

1.1

  1. *NTA is for not taken into account; TA is for taken into account; PD is for pairwise deletion, CD is for complete deletion