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Figure 4 | Frontiers in Zoology

Figure 4

From: Development of the retinotectal system in the direct-developing frog Eleutherodactylus coqui in comparison with other anurans

Figure 4

Cell proliferation during tectal development in E. coqui ( A - C ) and D. pictus ( D - F ) as revealed by immunostaining for proliferating cell nuclear antigen (PCNA) in transverse paraffine sections. The border of the optic tectum is indicated on the right side of each panel. Tectal layers are identified by numbers in panels C and F. At early embryonic stages of both species (A, D), the optic tectum is very small and all tectal cells are PCNA immunoreactive (orange or brown nuclei). At later embryonic stages (B, E), the tectum of E. coqui has enormously grown in size, while the tectum of D. pictus is still small (note different magnification). While the entire ventricular layer remains PCNA immunoreactive, the first non-proliferating cells are evident (arrows). At hatching, the tectum of E. coqui (C) resembles the tectum of D. pictus at an early larval stage (F): tectal layers 5 and 7–9 have differentiated, while PCNA immunopositive cells continue to be present along the entire ventricular surface. Bar: 100 μm in all panels.

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