Outlining eicosanoid biosynthesis in the crustacean Daphnia

Table 1 Putative Daphnia pulex genes associated with eicosanoid biosynthesis

Putative gene^a	Protein ID^b	Protein size (AA)	Best match (species)^c	Accession number	Similarity (%)	E-value
Phospholipase A2	DAPPUDRAFT_5959	129	Apis mellifera	XP_624621	76	0.0
Cyclooxygenase	DAPPUDRAFT_313427	689	*Sus scrofa*	NP_999486	62	0.0
Prostaglandin D2 synthase A	DAPPUDRAFT_307787	211	Tribolium castaneum	XP_970647	49	2.5E-35
Prostaglandin D2 synthase B	DAPPUDRAFT_316534	228	T. castaneum	XP_967406	54	2.3E-32
Prostaglandin E2 synthase	DAPPUDRAFT_56335	283	Aedes aegypti	EAT39424	61	0.0
Carbonyl reductase 1 (PG 9-ketoreductase)	DAPPUDRAFT_310758	257	*Xenopus laevis*	Q6DJN9	76	0.0
Thromboxane A	DAPPUDRAFT_96715	463	Homarus americanus	AAC28351	49	0.0
Thromboxane B	DAPPUDRAFT_328913	501	H. americanus	AAC28351	59	0.0
Lipoxygenase 1	DAPPUDRAFT_311736	562	T. castaneum	XP_969219	48	0.0
Lipoxygenase 2	DAPPUDRAFT_95367	433	*Bos taurus*	XP_593842	33	2.1E-43
Glutathione peroxidase	DAPPUDRAFT_337058	219	Ixodes scapularis	AAY66814	67	0.0
Leukotriene A4 hydrolase	DAPPUDRAFT_313156	619	*X. tropicalis*	NP_001006898	67	0.0
Leukotriene B4 12-hydroxydehydrogenase	DAPPUDRAFT_311788	340	A. aegypti	EAT35376	65	0.0
Prostanoid receptor EP4 isoform A	DAPPUDRAFT_58618	166	*Danio rerio*	LOC560057	59	3.6E-38
Prostanoid receptor EP4 isoform B	DAPPUDRAFT_58558	121	*Tetraodon nigroviridis*	CAF96390	60	4.2E-30

^aGene names displayed in italics are unlikely to be involved in daphnid eicosanoid biosynthesis (see Fig. 2 and text for further information); ^bD. pulex protein ID is linked to a GenBank [19] accession no.; ^cVertebrate species are signified by bold font, while invertebrate matches are denoted by normal font.

ISSN: 1742-9994