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Figure 5 | Frontiers in Zoology

Figure 5

From: Muscle formation during embryogenesis of the polychaete Ophryotrocha diadema (Dorvilleidae) – new insights into annelid muscle patterns

Figure 5

First chaetiger ciliary band – later stage. Scale bars = 50 μm, anterior is to the left. A, C, F. Phalloidin staining shown in red anti-acetylated tubulin staining in green, and nuclear staining in blue. Prototroch (prot), metatroch (met), ciliary band of peristomium (pcb), ciliary bands of chaetigers (BS) and telotroch (tel). Additional labeling as in B, E, H. Phalloidin staining. D, G. Phalloidin staining, depth coded (in μm). A. Same embryo as in B. Ventral view. The ventral longitudinal muscles (VLM) have elongated towards the pygidium. The first transverse muscles (arrow) differentiate. Mouth region (stom) clearly visible. C, D. Same Embryo as in E. Ventrolateral view. The dorsal (DLM) and ventral longitudinal muscles (VLM) have reached the pygidium. Adjacent to the autofluorescent chaetae (ch) lie transverse muscles, the anlage of the paraopodial muscles, laterally and ventrally (arrows). The ventral longitudinal muscles extend into the prostomium, forming three projections (dVLM, asterisk). The jaw apparatus (ja) is apparent ventral to the mouth opening (stom). F, G. Same Embryo as in H. Ventrolateral view. One transverse parapodial muscle anlage (arrow) is always present just anterior and just posterior of a ciliary band (pcb, BS). DLM-dorsal longitudinal muscle, VLM-ventral longitudinal muscle, ch-chaetae, stom-mouth opening.

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