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Figure 3 | Frontiers in Zoology

Figure 3

From: The exceptional stem cell system of Macrostomum lignano: Screening for gene expression and studying cell proliferation by hydroxyurea treatment and irradiation

Figure 3

Treatment of Macrostomum lignano with Hydroxyurea. (A) Non-treated control animal with BrdU-labeled S-phase cells arranged in two lateral bands (arrowheads). (e) alg remnants of digested food algae showing red autofluorescence, tp tail plate. (B) BrdU stainings of M. lignano treated with 2.5 mM Hydroxyurea for 8.5 days. Specimen shows about 10 BrdU-labelled cells in S-phase (arrowheads). Some background can be seen in the pharynx glands (pg) and the female genital opening (fgo). tp tail plate. scalebar 100 μm. (C) In situ hybridization of control animal with macvasa. Signal present in male (t testes) and female gonads (ov ovaries) and developing eggs (de). e eyes, tp tail plate. (D) In situ hybridization with macvasa after 11 days of HU-treatment. Only female gonads (ov ovaries) show signal. Male gonads (t testes) show no expression of macvasa. e eyes, de developing eggs, ov ovaries, t testes, scalebar 100 μm.

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Frontiers in Zoology

ISSN: 1742-9994