Confirmation of the enrichment of immune-related transcripts in the subtracted cDNA library by quantitative real-time PCR analysis. The relative amount of cDNAs of 18 S rRNA, α-tubulin, BPI, and SOCS in the subtracted cDNA library (white bars) is shown relative to their amount in unsubtracted cDNA library (black bars). The cDNA amount of the house-keeping genes 18 S rRNA and α-tubulin were reduced about 2.5 fold by the subtraction procedure. In contrast, potentially immunity-related genes BPI and SOCS were found to be 1.3 and 5.1 fold enriched in the subtracted cDNA library, respectively. Results represent mean values of three independent determinations ± S.D.