Figure 2

Anatomical details of the rhinophore. Neuroanatomy and histology of the rhinophore. A: Cross sections labelled with serotonin-immunoreactivity (green) and propidium iodide (red). Nuclear labelling shows the layers of cell nuclei in the sensory epithelium (SE) around the groove. Glomeruli (GL) are situated beneath the sensory epithelium. Scale bar = 500 μm. B, C, G: Histological cross sections of the rhinophore at different planes along the longitudinal axis (stained with Mallory's stain). The rhinophore ganglion (RG) is folded and located in the middle of the rhinophore. Scale bar = 500 μm. G: Higher magnification of the rhinophore ganglion shown in B. Scale bar = 100 μm. D, E, F,: Serotonin-immunoreactivity is labelled green and cell nuclei are labelled red in D and E, whereas in F phalloidin is labelled in red. The immunoreactivity is showing serotonergic innervation of the rhinophore ganglion (D, F) and the sensory epithelium (E). Cell nuclei in the sensory epithelium labelled with propidium iodide (D, E). F: Light labelling with phalloidin in the rhinophore ganglion. Scale bars for D, E, F, G = 100 μm. H: Injection of DiA (green) in the rhinophore ganglion revealed the connection from the rhinophore ganglion to a glomerulus. The big amount of DiA crystals are fluorescing yellow and indicate thereby the injection location. Labelling with Propidium iodide (red) shows the layers of cell nuclei in the epithelium. I: Histological section (Mallory stain). Individual glomerulus situated beneath the sensory epithelium and surrounded by a glial-like cell layer (arrow).